Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/01/2010
|
Resumo |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW (R) (Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 mu g/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC(50) values of 1.94 mu g/mL and 5.49 mu g/mL. Furthermore, it repressed T. cruzi growth with an IC(50) of 1.14 mu g/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi. |
Formato |
614-622 |
Identificador |
http://dx.doi.org/10.1590/S1678-91992010000400012 Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 4, p. 614-622, 2010. 1678-9199 http://hdl.handle.net/11449/22009 S1678-91992010000400012 WOS:000284778000012 S1678-91992010000400012-en.pdf |
Idioma(s) |
eng |
Publicador |
Universidade Estadual Paulista (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) |
Relação |
Journal of Venomous Animals and Toxins Including Tropical Diseases |
Direitos |
openAccess |
Palavras-Chave | #L-amino oxidase #Bothrops leucurus #antimicrobial activity |
Tipo |
info:eu-repo/semantics/article |