Bone regeneration in surgically created defects filled with autogenous bone: an epifluorescence microscopy analysis in rats


Autoria(s): Guskuma, Marcos Heidy; Hochuli-Vieira, Eduardo; Pereira, Flavia Priscila; Rangel-Garcia Junior, Idelmo; Okamoto, Roberta; Okamoto, Tetuo; Magro Filho, Osvaldo
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

30/09/2013

20/05/2014

30/09/2013

20/05/2014

01/07/2010

Resumo

Although the search for the ideal bone substitute has been the focus of a large number of studies, autogenous bone is still the gold standard for the filling of defects caused by pathologies and traumas, and mainly, for alveolar ridge reconstruction, allowing the titanium implants installation. Objectives: The aim of this study was to evaluate the dynamics of autogenous bone graft incorporation process to surgically created defects in rat calvaria, using epifluorescence microscopy. Material and methods: Five adult male rats weighing 200-300 g were used. The animals received two 5-mm-diameter bone defects bilaterally in each parietal bone with a trephine bur under general anesthesia. Two groups of defects were formed: a control group (n=5), in which the defects were filled with blood clot, and a graft group (n=5), in which the defects were filled with autogenous bone block, removed from the contralateral defect. The fluorochromes calcein and alizarin were applied at the 7th and 30th postoperative days, respectively. The animals were killed at 35 days. Results: The mineralization process was more intense in the graft group (32.09%) and occurred mainly between 7 and 30 days, the period labeled by calcein (24.66%). Conclusions: The fluorochromes showed to be appropriate to label mineralization areas. The interfacial areas between fluorochrome labels are important sources of information about the bone regeneration dynamics.

Formato

346-353

Identificador

http://dx.doi.org/10.1590/S1678-77572010000400005

Journal of Applied Oral Science. Bauru-sp: Univ São Paulo Fac Odontologia Bauru, v. 18, n. 4, p. 346-353, 2010.

1678-7757

http://hdl.handle.net/11449/15118

S1678-77572010000400005

WOS:000281692300005

S1678-77572010000400005-en.pdf

Idioma(s)

eng

Publicador

Universidade de São Paulo (USP), Faculdade de Odontologia de Bauru

Relação

Journal of Applied Oral Science

Direitos

openAccess

Palavras-Chave #Fluorescent dyes #Bone transplantation #Bone regeneration
Tipo

info:eu-repo/semantics/conferencePaper