Nicotine-Induced Damage Affects Gingival Fibroblasts in the Gingival Tissue of Rats


Autoria(s): Almeida, Juliano Milanezi de; Bosco, Álvaro Francisco; Bonfante, Samara; Theodoro, Letícia Helena; Nagata, Maria José Hitomi; Garcia, Valdir Gouveia
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

30/09/2013

20/05/2014

30/09/2013

20/05/2014

01/08/2011

Resumo

Background: Very limited information is available from in vivo studies about whether smoking and/or nicotine affect gingival tissues in the absence of plaque. The purpose of this study is to evaluate the effect of the systemic administration of nicotine in the proliferation and counting of fibroblast-like cells in the gingival tissue of rats.Methods: Thirty adult male Wistar rats were randomly assigned into two groups to receive subcutaneous injections of a saline solution (control group = group C) or nicotine solution (group N; 3 mg/kg) twice a day. The animals were euthanized 37, 44, or 51 days after the first subcutaneous injection. Specimens were routinely processed for serial histologic sections. Five fields of view in the connective tissue adjacent to the gingival epithelium and above the alveolar bone crest of the maxillary first molar were selected for the counting of fibroblast-like cells. Data were statistically analyzed (P<0.05).Results: The intergroup analysis detected a lower number of fibroblast-like cells in group N compared to group C on days 37 (2.65 +/- 1.41 and 6.67 +/- 3.25, respectively), 44 (2.70 +/- 1.84 and 8.57 +/- 2.37, respectively), and 51(2.09 +/- 1.41 and 7.49 +/- 2.60, respectively) (P<0.05). The quantification of fibroblast-like cells showed no significant difference (P >0.05) in the intragroup analysis of control and nicotine throughout experimental periods. In the intergroup analysis, group N had reduced proliferating cell nuclear antigen positive fibroblasts compared to group C in all periods (P<0.05).Conclusion: The daily systemic administration of nicotine negatively affected, in vivo, the number and proliferation of fibroblast-like cells in the gingival tissue of rats. J Periodontol 2011;82:1206-1211.

Formato

1206-1211

Identificador

http://dx.doi.org/10.1902/jop.2010.100549

Journal of Periodontology. Chicago: Amer Acad Periodontology, v. 82, n. 8, p. 1206-1211, 2011.

0022-3492

http://hdl.handle.net/11449/15080

10.1902/jop.2010.100549

WOS:000294080900013

Idioma(s)

eng

Publicador

Amer Acad Periodontology

Relação

Journal of Periodontology

Direitos

closedAccess

Palavras-Chave #Animal model #fibroblasts #nicotine #periodontitis
Tipo

info:eu-repo/semantics/article