Detection of Babesia bigemina in cattle of different genetic groups and in Rhipicephalus (Boophilus) microplus tick


Autoria(s): Oliveira, M. C. S.; Oliveira-Sequeira, T. C. G.; Regitano, L. C. A.; Alencar, M. M.; Neo, T. A.; Silva, A. M.; Oliveira, Henrique Nunes de
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

30/09/2013

20/05/2014

30/09/2013

20/05/2014

17/08/2008

Resumo

Babesia bigemina infections were investigated in four genetic groups of beef cattle and in Rhipicephalus (Boophilus) microplus engorged female ticks. Blood samples and engorged female ticks were collected from 15 cows and 15 calves from each of the following genetic groups: Nelore, Angus x Nelore, Canchim x Nelore, and Simmental x Nelore. Microscopic examination of blood smears and tick hemolymph revealed that merozoites of B. bigemina (6/60) as well as kinetes of Babesia spp. (9/549) were only detected in samples (blood and ticks, respectively) originated from calves. PCR-based methods using primers for specific detection of B. bigemina revealed 100% infection in both calves and cows, regardless the genetic group. Tick infection was detected by nested-PCR amplifications showing that the frequency of B. bigemina was higher (P 0.01) in female ticks collected from calves (134/549) than in those collected from cows (52/553). The frequency of B. bigemina was similar in ticks collected from animals, either cows or calves, of the four genetic groups (P > 0.05). (C) 2008 Elsevier B.V. All rights reserved.

Formato

281-286

Identificador

http://dx.doi.org/10.1016/j.vetpar.2008.04.022

Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 155, n. 3-4, p. 281-286, 2008.

0304-4017

http://hdl.handle.net/11449/14047

10.1016/j.vetpar.2008.04.022

WOS:000258361400015

Idioma(s)

eng

Publicador

Elsevier B.V.

Relação

Veterinary Parasitology

Direitos

closedAccess

Palavras-Chave #beef cattle #tick #babesiosis #PCR
Tipo

info:eu-repo/semantics/article