Killing of Paracoccidioides brasiliensis yeast cells by IFN-gamma and TNF-alpha activated murine peritoneal macrophages: evidence of H(2)O(2) and NO effector mechanisms
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/07/2008
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Resumo |
Paracoccidioidomycosis is a deep mycosis, endemic in Latin America, caused by Paracoccidioides brasiliensis. Macrophage activation by cytokines is the major effector mechanism against this fungus. This work aimed at a better understanding of the interaction between yeast cells-murine peritoneal macrophages and the cytokine signals required for the effective killing of high virulence yeast-form of P. brasiliensis. In addition, the killing effector mechanisms dependent on the generation of reactive oxygen or nitrogen intermediates were investigated. Cell preincubation with IFN-gamma or TNF-alpha, at adequate doses, resulted in effective yeast killing as demonstrated in short-term (4-h) assays. Both, IFN-gamma and TNF-alpha activation were associated with higher levels of H(2)O(2) and NO when compared to nonactivation. Treatment with catalase (CAT), a H(2)O(2) scavenger, and N(G)-monomethyl-L-arginine (L-NMMA), a nitric oxide synthase inhibitor, reverted the killing effect of activated cells. Taken together, these results suggest that both oxygen and L-arginine-nitric oxide pathways play a role in the killing of highly virulent P. brasiliensis. |
Formato |
17-23 |
Identificador |
http://dx.doi.org/10.1007/s11046-007-9046-3 Mycopathologia. Dordrecht: Springer, v. 166, n. 1, p. 17-23, 2008. 0301-486X http://hdl.handle.net/11449/11838 10.1007/s11046-007-9046-3 WOS:000257215400002 |
Idioma(s) |
eng |
Publicador |
Springer |
Relação |
Mycopathologia |
Direitos |
closedAccess |
Palavras-Chave | #Paracoccidioidomycosis #murine macrophages #fungicidal activity #cytokines #NO #H(2)O(2) |
Tipo |
info:eu-repo/semantics/article |