Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)


Autoria(s): Meira, D. A.; Almeida, Ricardo Augusto Monteiro de Barros; Barbosa, Alexandre Naime; Souza, Lenice do Rosario de; Olivo, T. E. T.; Henriques, R. M. S.; Golim, Márjorie de Assis; Araujo Jr, J. P.; Nagoshi, L. R.; Orikaza, C. M.; Calvi, Sueli Aparecida
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/01/2008

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G(1) = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G(2) = 27); and patients on HAART with undetectable VL for at least the past six months (G(3) = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G(4) = 20), which was the control group. Serum cytokine levels (values in pg/mL) were measured by enzyme-linked immunosorbent assay (ELISA) and specific mRNA expression by reverse transcription polymerase chain reaction (RT-PCR). Both techniques were performed on the four groups for TNF-alpha, IL-2, INF-gamma, IL-4 and IL-10. All patients were submitted to VL determination and CD4(+) and CD8(+) T lymphocyte counts. The analysis of the results revealed a significant comparison among groups for both methods and an association between the latter (>80% - r(2) > 0.80). There was only one exception, in control individuals for IL-2 by ELISA. The cytokine profiles, in both methods, for the three patient groups, were mature Th-0. The behaviors of IL-2 and INF-gamma required emphasis due to consequent expression of dominant Th profile. Both methods showed low IL-2 and high mean values of INF-gamma in the three groups. Several authors have recently drawn attention to the substantial apoptosis of infected and non-infected CD4(+) T cells, mainly during primary infection, persisting only in those with INF-gamma phenotype producer and not IL-2. HIV infected individuals submitted to HAART are expected to produce IL-2 in an attempt to present Th-1 profile, but in most cases this did not occur.

Formato

685-702

Identificador

http://dx.doi.org/10.1590/S1678-91992008000400011

Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 14, n. 4, p. 685-702, 2008.

1678-9199

http://hdl.handle.net/11449/11806

S1678-91992008000400011

WOS:000267352500011

S1678-91992008000400011-en.pdf

Idioma(s)

eng

Publicador

Universidade Estadual Paulista (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)

Relação

Journal of Venomous Animals and Toxins Including Tropical Diseases

Direitos

openAccess

Palavras-Chave #RT-PCR #ELISA #cytokines #HIV #AIDS #HAART #apoptosis
Tipo

info:eu-repo/semantics/article