Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/09/2011
|
Resumo |
Phenotypic and genotypic SPM and IMP metallo-beta-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either bla(SPM) or bla(IMP) genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of bla(SPM) and bla(IMP) genes among Brazilian clinical isolates. |
Formato |
478-481 |
Identificador |
http://dx.doi.org/10.1590/S1413-86702011000500011 Brazilian Journal of Infectious Diseases. Salvador: Contexto, v. 15, n. 5, p. 478-481, 2011. 1413-8670 http://hdl.handle.net/11449/11437 S1413-86702011000500011 WOS:000295936600011 S1413-86702011000500011-en.pdf |
Idioma(s) |
eng |
Publicador |
Contexto |
Relação |
Brazilian Journal of Infectious Diseases |
Direitos |
openAccess |
Palavras-Chave | #Pseudomonas aeruginosa #drug resistance #bacterial #carbapenems #metalloproteins #polymerase chain reaction |
Tipo |
info:eu-repo/semantics/article |