Absence of mutagenicity effects of Psidium cattleyanum Sabine (Myrtaceae) extract on peripheral blood and bone marrow cells of mice
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/01/2008
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Resumo |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Processo FAPESP: 06/57514-2 Cattley guava (Psidium cattleyanum Sabine) is a native fruit of Brazil that is popular both as a sweet food and for its reputed therapeutic properties. We examined whether it could damage DNA using the alkaline single-cell gel electrophoresis (comet assay) and the micronucleus test in leukocytes and in bone marrow cells of mice. P. cattleyanum leaf extract was tested at concentrations of 1000, 1500 and 2000 mg/kg. N-nitroso-N-ethylurea was used as a positive control. Peripheral blood leukocytes were collected 4 and 24 h after the treatments for the comet assay, and bone marrow cells were collected after 24 and 48 h for the micronucleus test. Unlike N-nitroso-N-ethylurea, P. cattleyanum extract failed to induce a significant increase in cell DNA damage, in micronucleated cell frequency, and in bone marrow toxicity. The lack of mutagenicity and cytotoxicity with high doses of this plant extract means that it can be safely used in traditional medicine. |
Formato |
679-686 |
Identificador |
http://dx.doi.org/10.4238/vol7-3gmr475 Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 7, n. 3, p. 679-686, 2008. 1676-5680 http://hdl.handle.net/11449/10806 10.4238/vol7-3gmr475 WOS:000260227100011 WOS000260227100011.pdf |
Idioma(s) |
eng |
Publicador |
Funpec-editora |
Relação |
Genetics and Molecular Research |
Direitos |
openAccess |
Palavras-Chave | #Psidium cattleyanum #Comet assay #Micronucleus test #Single-cell gel electrophoresis #Mutagenesis |
Tipo |
info:eu-repo/semantics/article |