Fibroblast functionality on novel Ti-30Ta nanotube array

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

In this study, the mechanical substrate and topographical surface properties of anodized Ti - 30Ta alloy were investigated using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and contact angle measurement. The anodization process was performed in an electrolyte solution containing HE (48%) and H2SO4 (98%) in the volumetric ratios 1:9 with the addition of 5% dimethyl sulfoxide (DMSO) at 15 V, 25 V and 35 V for 20 and 40 min, producing a nanotube architecture when anodized at 35 V for 40 min. Human dermal fibroblasts (HDF, neonatal) were utilized to evaluate the biocompatibility of Ti - 30Ta nanotubes and Ti - 30Ta alloy after 1 and 3 days of culture. Cellular adhesion, proliferation, viability, cytoskeletal organization and morphology were investigated using fluorescence microscope imaging, biochemical assay and SEM imaging respectively. The results presented identify altered material properties and improved cellular interaction on Ti - 30Ta nanotubes as compared to Ti - 30 Ta alloy. (C) 2012 Elsevier B.V. All rights reserved.