Molecular differentiation of Salmonella Gallinarum and Salmonella Pullorum by RFLP of fliC gene from Brazilian isolates
Contribuinte(s) |
Universidade Estadual Paulista (UNESP) |
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Data(s) |
20/05/2014
20/05/2014
01/12/2009
|
Resumo |
Although Salmonella Pullorum and Salmonella Gallinarum cause different diseases in poultry, they are very similar. Both are non-motile and present the same somatic antigenic structure. They are differentiated by biochemical tests. Certain atypical strains are very difficult to distinguish. They do not produce the expected results when dulcitol and ornithine descarxboxylase tests are performed. Therefore, additional tests could be helpful. Many studies have chose the part I of the gene that encodes flagellin (fliC) to differentiate serotypes. Most Salmonella strains have two structural genes (fliC and fliB) that encode flagellins. Non-motile strains generally present these structural genes, but are not able to build a functional flagellum. It was demonstrated that enzymatic restriction of the amplified fliC gene using Hinp1I enzyme can differentiate SG from SP. In the present study, this method was adopted to analyze 14 SP and 22 SG strains, including some strains with atypical results in biochemical tests assessing the utilization of dulcitol and ornithine. The results showed that all SG strains were broken by the enzyme, whereas the 14 SP strains were not. |
Formato |
271-275 |
Identificador |
http://dx.doi.org/10.1590/S1516-635X2009000400009 Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 11, n. 4, p. 271-275, 2009. 1516-635X http://hdl.handle.net/11449/3107 10.1590/S1516-635X2009000400009 S1516-635X2009000400009 WOS:000276475000009 S1516-635X2009000400009.pdf |
Idioma(s) |
eng |
Publicador |
Fundação APINCO de Ciência e Tecnologia Avícolas |
Relação |
Revista Brasileira de Ciência Avícola |
Direitos |
openAccess |
Palavras-Chave | #FliC gene #Differentiation #Salmonella Gallinarum #Salmonella Pullorum |
Tipo |
info:eu-repo/semantics/article |