Purification and biological effects of a C-type lectin isolated from Bothrops moojeni


Autoria(s): Barbosa, P. S. F.; Martins, A. M. C.; Toyama, M. H.; Joazeiro, P. P.; Beriam, L. O. S.; Fonteles, M. C.; Monteiro, H. S. A.
Contribuinte(s)

Universidade Estadual Paulista (UNESP)

Data(s)

20/05/2014

20/05/2014

01/01/2010

Resumo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and investigated its effect on platelet aggregation, insulin secretion, antibacterial activity, and isolated kidney cells. The BmLec was purified using two chromatographic steps: affinity chromatography and reverse phase high performance liquid chromatography (HPLC). BmLec showed a dose-dependent platelet aggregation and significantly decreased the bacterial growth rate in approximately 15%. During scanning electron microscopy, the profile of Xanthomonas axonopodis pv. passiflorae treated with lectin disclosed a high vesiculation and membrane rupture. BmLec induced a strong and significant increase in insulin secretion at 2.8 and 16.7 mM glucose concentrations, and this effect was seen in the presence of EGTA in both experiments. BmLec (10 mu g/mL) increased the perfusion pressure, renal vascular resistance and urinary flow. The glomerular filtration rate and percentages of sodium, potassium and chloride tubular transport were reduced at 60 minutes of perfusion. Renal alterations caused by BmLec were completely inhibited by indomethacin in all evaluated parameters. In conclusion, the C-type lectin isolated from Bothrops moojeni affected platelet aggregation, insulin secretion, antibacterial activity and isolated kidney function.

Formato

493-504

Identificador

http://dx.doi.org/10.1590/S1678-91992010000300016

Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 3, p. 493-504, 2010.

1678-9199

http://hdl.handle.net/11449/329

S1678-91992010000300016

WOS:000281692600016

S1678-91992010000300016-en.pdf

Idioma(s)

eng

Publicador

Universidade Estadual Paulista (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)

Relação

Journal of Venomous Animals and Toxins Including Tropical Diseases

Direitos

openAccess

Palavras-Chave #Bothrops moojeni #kidney #platelet aggregation #insulin #antibacterial activity
Tipo

info:eu-repo/semantics/conferencePaper