Prevention of bacterial translocation using β-(1-3)-D-glucan in small bowel ischemia and reperfusion in rats


Autoria(s): Araújo Filho, Irami; Rêgo, Amália Cínthia Meneses; Pinheiro, Laíza Araújo Mohana; Azevedo, Italo Medeiros; Medeiros, Vítor Brasil; Brandão Neto, José; Medeiros, Aldo Cunha
Data(s)

21/10/2013

21/10/2013

2006

Resumo

To investigate the role of β-(1-3)-D-glucan on 99mTc labelled Escherichia coli translocation and cytokines secretion in rats submitted to small bowel ischemia/reperfusion injury. Methods: Five groups (n=10 each) of Wistar rats were subjected to control(C), sham(S), group IR subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R), and group I/R+glucan subjected to 45 min of bowel ischemia/60 min of reperfusion(I/R) and injected with 2mg/Kg intramuscular. Translocation of labelled bacteria to mesenteric lymph nodes, liver, spleen, lung and serum was determined using radioactivity/count and colony forming units/g(CFU/g). Serum TNFα, IL-1β, IL-6, IL-10 were measured by ELISA. Results: CFU/g and radioactivity/count were higher in I/R than in I/R+glucan rats. In C, S and S+glucan groups, bacteria and radioactivity/count were rarely detected. The I/R+glucan rats had enhancement of IL-10 and suppressed production of serum TNFα, IL-1β and, IL-6, compared to I/R untreated animals. Conclusion: The β-(1-3)-D-glucan modulated the production of pro-inflammatory and anti-inflammatory cytokines during bowel ischemia/reperfusion, and attenuated translocation of labelled bacteria

CNPq

Identificador

ARAÚJO FILHO, Irami ; RÊGO, Amália Cínthia Meneses ; PINHEIRO, Laíza Araújo Mohana ; AZEVEDO, Italo Medeiros ; MEDEIROS, Vítor Brasil ; BRANDÃO NETO, José ; MEDEIROS, Aldo Cunha . Prevention of bacterial translocation using beta-(1-3)-D-glucan in small bowel ischemia and reperfusion in rats. Acta Cirúrgica Brasileira , v. 21, p. 18-22, 2006. Disponível em: <http://www.scielo.br/scielo.php?pid=S0102-86502006001000005&script=sci_arttext> Acesso em: 09 out. 2013.

1678-2674

http://repositorio.ufrn.br:8080/jspui/handle/1/6617

Idioma(s)

eng

Direitos

open access

Palavras-Chave #Bacterial translocation #Glucan #Intestine #Ischemia #Reperfusion #Prophylaxis
Tipo

article