Cross-platform urine metabolomics of experimental hyperglycemia in type 2 diabetes
Data(s) |
01/01/2012
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Resumo |
Hyperglycemia causes diabetic nephropathy, a condition for which there are no specific diagnostic markers thatpredict progression to renal failure. Here we describe a multiplatform metabolomic analysis of urine from individualswith type 2 diabetes, collected before and immediately following experimental hyperglycemia. We used targetednuclear magnetic resonance spectroscopy (NMR), liquid chromatography - mass spectrometry (LC-MS) and gaschromatography - MS (GC-MS) to identify markers of hyperglycemia. Following optimization of data normalisation andstatistical analysis, we identified a reproducible NMR and LC-MS based urine signature of hyperglycemia. Significantincreases of alanine, alloisoleucine, isoleucine, leucine, N-isovaleroylglycine, valine, choline, lactate and taurine anddecreases of arginine, gamma-aminobutyric acid, hippurate, suberate and N-acetylglutamate were observed. GC-MSanalysis identified a number of metabolites differentially present in post-glucose versus baseline urine, but these could not be identified using current metabolite libraries. This analysis is an important first step towards identifying biomarkers of early-stage diabetic nephropathy. |
Identificador | |
Idioma(s) |
eng |
Publicador |
OMICS Publishing Group |
Relação |
http://dro.deakin.edu.au/eserv/DU:30083267/callahan-crossplatform-2012.pdf http://www.dx.doi.org/10.4172/2155-6156.S6-002 |
Direitos |
2012, OMICS Publishing Group |
Palavras-Chave | #Metabolomics #Urine #Hyperglycemia #Hyperglycaemia #Diabetes #NMR #GC #LC #MS #Biomarker |
Tipo |
Journal Article |