A computational search for lipases that can preferentially hydrolyze long-chain omega-3 fatty acids from fish oil triacylglycerols
Data(s) |
15/04/2015
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Resumo |
Consumption of long-chain omega-3 fatty acids is known to decrease the risk of major cardiovascular events. Lipases, a class of triacylglycerol hydrolases, have been extensively tested to concentrate omega-3 fatty acids from fish oils, under mild enzymatic conditions. However, no lipases with preference for omega-3 fatty acids selectivity have yet been discovered or developed. In this study we performed an exhaustive computational study of substrate-lipase interactions by docking, both covalent and non-covalent, for 38 lipases with a large number of structured triacylglycerols containing omega-3 fatty acids. We identified some lipases that have potential to preferentially hydrolyze omega-3 fatty acids from structured triacylglycerols. However omega-3 fatty acid preferences were found to be modest. Our study provides an explanation for absence of reports of lipases with omega-3 fatty acid hydrolyzing ability and suggests methods for developing these selective lipases. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier |
Relação |
http://dro.deakin.edu.au/eserv/DU:30070982/barrow-acomputational-2015.pdf |
Direitos |
2015, Elsevier |
Palavras-Chave | #Covalent docking #DHA #EPA #Lipase #Omega-3 fatty acids #Science & Technology #Physical Sciences #Life Sciences & Biomedicine #Chemistry, Applied #Food Science & Technology #Nutrition & Dietetics #Chemistry #POLYUNSATURATED FATTY-ACIDS #CARDIOVASCULAR-DISEASE #OPTIMIZATION |
Tipo |
Journal Article |