A non-destructive test to assess the axial heterogeneity of in situ modified monoliths for HPLC
Data(s) |
01/01/2015
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Resumo |
This paper describes a non-destructive "peak parking" protocol in order to assess the axial heterogeneity of an in situ modified monolithic column for high performance liquid chromatography; a "gradient stationary phase" was designed whereby the ligand density decreases along the length of the rod in the "forward flow" configuration. The results of multi-location peak parking demonstrated a consistent increase in peak variance from the 1 cm position of the column to the 9 cm location. This increase in band broadening supported the theory of a decreasing ligand density along the length of this gradient column. This is consistent with efficiency measurements performed in both the forward and reverse flow directions, with an improved efficiency (15% increase in N m<sup>-1</sup>) in the reverse direction. These results are consistent with theoretical investigations into stationary phase gradients. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Royal Society of Chemistry |
Relação |
http://dro.deakin.edu.au/eserv/DU:30078256/bassanese-anondestructivetest-2015.pdf http://www.dx.doi.org/10.1039/c4ay02812k |
Direitos |
2015, Royal Society of Chemistry |
Tipo |
Journal Article |