Synthesis of ethyl laurate by hydrogel immobilized lipase of Bacillus coagulans MTCC-6375
Data(s) |
01/01/2005
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Resumo |
An alkaline thermo-tolerant lipase from Bacillus coagulans MTCC-6375 was purified and efficiently immobilized onto a synthetic hydrophobic poly (MAc-co-DMA-cl-MBAm)-hydrogel at pH 8.5 and temperature 55°C in 16 h. The hydrogel bound matrix possessed 7.6 IU g -1 matrix lipase activity with a specific activity of 18 IU mg -1 protein. Immobilized lipase was used to catalyze the esterification of lauric acid and ethanol to produce ethyl laurate in n-nonane. The reaction conditions that were optimized to produce ethyl laurate in n-nonane included enzyme/substrate (E/S) ratio, substrate concentration, reaction time and reaction temperature. The optimized parameters were E/S ratio of 0.5 mg mM -1, ethanol:lauric acid in ratio of 100 mM:100 mM and reaction time of 15 h at 65°C under continuous shaking (200 rpm). Optimized conditions resulted in 66% conversion of reactants into ethyl laurate in n-nonane in the presence of 300 mg molecular sieve mL -1 reaction mixture. |
Identificador | |
Idioma(s) |
eng |
Publicador |
Springer (India) Private |
Palavras-Chave | #Bacillus coagulans #Ethyl laurate synthesis #Lipase esterification #Poly (MAc-co-DMA-cl-MBAm)-hydrogel |
Tipo |
Journal Article |