Gag-Pol supplied in trans is efficiently packaged and supports viral function in human immunodeficiency virus type 1
Data(s) |
01/08/2001
|
---|---|
Resumo |
The intracellular trafficking and subsequent incorporation of Gag-Pol into human immunodeficiency virus type 1 (HIV-1) remains poorly defined. Gag-Pol is encoded by the same mRNA as Gag and is generated by ribosomal frameshifting. The multimerization of Gag and Gag-Pol is an essential step in the formation of infectious viral particles. In this study, we examined whether the interaction between Gag and Gag-Pol is initiated during protein translation in order to facilitate the trafficking and subsequent packaging of Gag-Pol into the virion. A conditional cotransfection system was developed in which virion formation required the coexpression of two HIV-1-based plasmids, one that produces both Gag and Gag-Pol and one that only produces Gag-Pol. The Gag-Pol proteins were either immunotagged with a His epitope or functionally tagged with a mutation (K65R) in reverse transcriptase that is associated with drug resistance. Gag-Pol packaging was assessed to determine whether the Gag-Pol incorporated into the virion was preferentially packaged from the plasmid that expressed both Gag and Gag-Pol or whether it could be packaged from either plasmid. Our data show that translation of Gag and Gag-Pol from the same mRNA is not critical for virion packaging of the Gag-Pol polyprotein or for viral function.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
American Society for Microbiology |
Relação |
http://dro.deakin.edu.au/eserv/DU:30047532/mak-gogpolsupplied-2001.pdf http://dx.doi.org/10.1128/JVI.75.15.6835-6840.2001 |
Direitos |
2001, American Society for Microbiology |
Palavras-Chave | #fusion proteins #gag-pol #gene products #gag #HIV-1 #protein biosynthesis #RNA #messenger #viral RNA #genetic transcription #transfection #virus assembly |
Tipo |
Journal Article |