Effect of naloxone-precipitated morphine withdrawal on c-fos expression in rat corticotropin-releasing hormone neurons in the paraventricular hypothalamus and extended amygdala
Data(s) |
13/05/2004
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Resumo |
Morphine withdrawal is characterized by physical symptoms and a negative affective state. The 41 amino acid polypeptide corticotropin-releasing hormone (CRH) is hypothesized to mediate, in part, both the negative affective state and the physical withdrawal syndrome. Here, by means of dual-immunohistochemical methodology, we examined the co-expression of the c-Fos protein and CRH following naloxone-precipitated morphine withdrawal. Rats were treated with slow-release morphine 50 mg/kg (subcutaneous, s.c.) or vehicle every 48 h for 5 days, then withdrawn with naloxone 5 mg/kg (s.c.) or saline 48 h after the final morphine injection. Two hours after withdrawal rats were perfused transcardially and their brains were removed and processed for immunohistochemistry. We found that naloxone-precipitated withdrawal of morphine-dependent rats increased c-Fos immunoreactivity (IR) in CRH positive neurons in the paraventricular hypothalamus. Withdrawal of morphine-dependent rats also increased c-Fos-IR in the central amygdala and bed nucleus of the stria terminalis, however these were in CRH negative neurons.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier Ireland |
Relação |
http://dro.deakin.edu.au/eserv/DU:30044492/day-effectofnaloxone-2004.pdf http://dx.doi.org/10.1016/j.neulet.2004.02.033 |
Direitos |
2004, Elsevier Ireland |
Palavras-Chave | #morphine withdrawal #c-fos #corticotropin-releasing hormone #paraventricular hypothalamus #amygdala #immunohistochemistry |
Tipo |
Journal Article |