Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen : intracellular targeting to the amyloplast
Data(s) |
01/02/1991
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Resumo |
We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain. |
Identificador | |
Idioma(s) |
eng |
Publicador |
National Academy of Sciences |
Relação |
http://dro.deakin.edu.au/eserv/DU:30039874/taylor-isolationof-1991.pdf http://dx.doi.org/10.1073/pnas.88.4.1384 |
Palavras-Chave | #IgE binding #molecular cloning #Lolium perenne #Lol pI allergen #immunogold probes |
Tipo |
Journal Article |