Identification and characterization of genes conferring salt tolerance to Escherichia coli from pond water metagenome
Data(s) |
01/06/2010
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Resumo |
Metagenomics provides culture-independent access to gene pool of the whole microbial communities. To identify genes responsible for salt tolerance in unculturable bacteria, <i>Escherichia coli</i> clones were enriched with an ability to grow at inhibitory NaCl concentrations (750 mM) from a pond water metagenomic library. From two unique clones, genes encoding for proteins with similarity to a putative general stress protein (GspM) harbouring GsiB domain and a putative enoyl-CoA hydratase (EchM) were identified to be responsible for salt tolerance. The <i>gspM</i> was expressed by its native promoter whereas the echM was expressed from the <i>lacZ</i> promoter of the plasmid. EchM was overexpressed with a hexahistidyl tag. Purified EchM showed crotonyl-CoA hydratase activity. These genes have potential application in generating salt tolerant recombinant bacteria or transgenic plants.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Elsevier |
Relação |
http://dro.deakin.edu.au/eserv/DU:30023627/puri-identicationandcharacterization-2010.pdf http://dx.doi.org/10.1016/j.biortech.2010.01.017 |
Direitos |
2010, Elsevier Ltd. |
Palavras-Chave | #metagenome #salt tolerance #Enoyl-CoA hydratase #general stress protein |
Tipo |
Journal Article |