Chemical characterisation of the oligosaccharides in hooded seal (Cystophora cristata) and Australian fur seal (Arctocephalus pusillus doriferus) milk
Data(s) |
01/02/2001
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Resumo |
Carbohydrates were extracted from hooded seal milk, <i>Crystophora cristata</i> (family Phocidae). Free oligosaccharides were separated by gel filtration and then purified by ion exchange chromatography, gel filtration and preparative thin layer or paper chromatography and their structures determined by <sup>1</sup>H-NMR. The hooded seal milk was found to contain inositol and at least nine oligosaccharides, most of which had lacto-<i>N</i>-neotetraose or lacto-N-neohexaose as core units, similar to those in milk of other species of Carnivora such as bears (Ursidae). Their structures were as follows: Gal(β1-4)Glc (lactose); Fuc(α1-2)Gal(β1-4)Glc (2′-fucosyllactose); Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (lacto-<i>N</i>-neotetraose); Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (lacto-<i>N</i>-fucopentaose IV); Gal(β1-4)GlcNAc(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(1-4)Glc (lacto-<i>N</i>-neohexaose); Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)[Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (monofucosyl lacto-<i>N</i>-neohexaose a); Gal(β1-4)GlcNAc(β1-3)[Fuc(α1-2)Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (monofucosyl lacto-<i>N</i>-neohexaose b); Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)[Fuc(α1-2)Gal(β1-4)GlcNAc(β1-6)]Gal(β1-4)Glc (difucosyl lacto-<i>N</i>-neohexaose); Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (<i>para</i> lacto-<i>N</i>-neohexaose); Fuc(α1-2)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)GlcNAc(β1-3)Gal(β1-4)Glc (monofucosyl para lacto-<i>N</i>-neohexaose). Milk of the Australian fur seal, <i>Arctophalus pusillus doriferus </i>(family Otariidae) contained inositol but no lactose or free oligosaccharides. These results, therefore, support the hypothesis that the milk of otariids, unlike that of phocids, contains no free reducing saccharides.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Pergamon Press |
Relação |
http://dx.doi.org/10.1016/S1096-4959(00)00327-4 |
Direitos |
2001, Elsevier Science Inc. |
Palavras-Chave | #hooded seal #milk #oligosaccharides #chemical structure #cystophora cristata #phocidae #Australian fur seal #otariidae |
Tipo |
Journal Article |