Perinatal ω-3 polyunsaturated fatty acid supply modifies brain zinc homeostasis during adulthood
Data(s) |
17/05/2005
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Resumo |
Dietary ω-3 polyunsaturated fatty acid (PUFA) influences the expression of a number of genes in the brain. Zinc transporter (ZnT) 3 has been identified as a putative transporter of zinc into synaptic vesicles of neurons and is found in brain areas such as hippocampus and cortex. Neuronal zinc is involved in the formation of amyloid plaques, a major characteristic of Alzheimer's disease. The present study evaluated the influence of dietary ω-3 PUFA on the expression of the ZnT3 gene in the brains of adult male Sprague-Dawley rats. The rats were raised and/or maintained on a control (CON) diet that contained ω-3 PUFA or a diet deficient (DEF) in ω-3 PUFA. ZnT3 gene expression was analyzed by using real-time PCR, free zinc in brain tissue was determined by zinquin staining, and total zinc concentrations in plasma and cerebrospinal fluid were determined by atomic absorption spectrophotometry. Compared with CON-raised animals, DEF-raised animals had increased expression of ZnT3 in the brain that was associated with an increased level of free zinc in the hippocampus. In addition, compared with CON-raised animals, DEF-raised animals had decreased plasma zinc level. No difference in cerebrospinal fluid zinc level was observed. The results suggest that overexpression of ZnT3 due to a perinatal ω-3 PUFA deficiency caused abnormal zinc metabolism in the brain. Conceivably, the influence of dietary ω-3 PUFA on brain zinc metabolism could explain the observation made in population studies that the consumption of fish is associated with a reduced risk of dementia and Alzheimer's disease.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
National Academy of Sciences |
Relação |
http://dro.deakin.edu.au/eserv/DU:30008874/perinatalw-3-2005.pdf http://dx.doi.org/10.1073/pnas.0502594102 |
Direitos |
2005, National Academy of Science |
Palavras-Chave | #essential fatty acid deficiency #zinc transporter #gene expression #docosahexaenoic acid #Alzheimer's disease |
Tipo |
Journal Article |