The brachyspira hyodysenteriae ftnA gene : DNA vaccination and real-time PCR quantification of bacteria in a mouse model of disease
Data(s) |
01/06/2005
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Resumo |
The nucleotide sequence of the <i>Brachyspira hyodysenteriae ftnA</i> gene, encoding a putative ferritin protein (FtnA), was determined. Analysis of the sequence predicted that this gene encoded a protein of 180 amino acids. RT-PCR and Western blot showed that the <i>ftnA</i> gene was expressed in <i>B.</i> <i>hyodysenteriae</i>, and evidence suggests that FtnA stores iron rather than haem. ftnA was delivered as DNA and recombinant protein vaccines in a mouse model of <i>B. hyodysenteriae</i> infection. Vaccine efficacy was monitored by caecal pathology and quantification of <i>B. hyodysenteriae</i> numbers in the caeca of infected mice by real-time PCR.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Springer New York LLC |
Relação |
http://dro.deakin.edu.au/eserv/DU:30006509/n20051462.pdf http://dx.doi.org/10.1007/s00284-005-4472-2 |
Direitos |
2005, Springer Science + Business Media Ιnc. |
Tipo |
Journal Article |