Identification and characterization of a lymphocytic Rho-GTPase effector: rhotekin-2
Data(s) |
26/11/2004
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Resumo |
Rhotekin belongs to the group of proteins containing a Rho-binding domain that are target peptides (effectors) for the Rho-GTPases. We previously identified a novel cDNA with homology to human rhotekin and in this study we cloned and characterized the coding region of this novel 12-exon gene. The ORF encodes a 609 amino-acid protein comprising a Class I Rho-binding domain and pleckstrin homology (PH) domain. Cellular cDNA expression of this new protein, designated Rhotekin-2 (RTKN2), was shown in the cytosol and nucleus of CHO cells. Using bioinformatics and RTPCR we identified three major splice variants, which vary in both the Rho-binding and PH domains. Real-time PCR studies showed exclusive RTKN2 expression in pooled lymphocytes and further purification indicated sole expression in CD4<sup><sub>pos</sub></sup> T-cells and bone marrow-derived B-cells. Gene expression was increased in quiescent T-cells but negligible in activated proliferating cells. In malignant samples expression was absent in myeloid leukaemias, low in most B-cell malignancies and CD8<sup>pos</sup> T-cell malignancies, but very high in CD4<sup>pos</sup>/CD8<sup>pos</sup> T-lymphoblastic lymphoma. As the Rho family is critical in lymphocyte development and function, RTKN2 may play an important role in lymphopoiesis.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Academic Press |
Relação |
http://dro.deakin.edu.au/eserv/DU:30002445/n20040301.pdf http://dx.doi.org/10.1016/j.bbrc.2004.09.205 |
Direitos |
2004, Elsevier Inc. |
Palavras-Chave | #Rhotekin #Lymphocytes #T-cells #Rho-GTPases #splice variants |
Tipo |
Journal Article |