Human skeletal muscle creatine transporter mRNA and protein expression in healthy, young males and females
Data(s) |
01/02/2003
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Resumo |
The present study investigated whether there were any differences between males and females in respect to creatine transporter (CreaT) gene expression and/or total creatine (TCr) content in human vastus lateralis muscle. Skeletal muscle obtained from young healthy male (n = 13, age: 23.2 ± 5.0 years) and female subjects (n = 12, age: 21.7 ± 4.3 years) was analyzed for CreaT mRNA, CreaT protein and TCr content. Total CreaT protein content in the muscle was similar (p > 0.05) between the sexes. Two bands (~ 55 and 73 kDa) of the CreaT protein were detected in all muscle samples. Both the 55 and the 73 kDa bands were present in similar (p > 0.05) amounts in males compared with females. The 73 kDa band was in greater abundance (p < 0.05) than the 55 kDa band, irrespective of gender. In addition, CreaT mRNA expression relative to <i>ß</i>-actin mRNA and the TCr content (males: 117.8 ± 2.2, females: 125.3 ± 4.3 mmol.kg<sup>–1</sup> dry mass) were also unaffected (p > 0.05) by gender. These data demonstrate that gender does not influence skeletal muscle TCr content and CreaT gene expression in young human subjects.<br /><br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
Springer Netherlands |
Relação |
http://dro.deakin.edu.au/eserv/DU:30002223/n20031055.pdf http://dx.doi.org/10.1023/A:1022495520722 |
Direitos |
2003 Kluwer Academic Publishers |
Palavras-Chave | #creatine metabolism #gene expression #high-energy phosphates |
Tipo |
Journal Article |