A short-term, high-fat diet up-regulates lipid metabolism and gene expression in human skeletal muscle
Data(s) |
01/02/2003
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Resumo |
<b>Background</b>: Dietary fatty acids may be important in regulating gene expression. However, little is known about the effect of changes in dietary fatty acids on gene regulation in human skeletal muscle. <br /><b>Objective</b>: The objective was to determine the effect of altered dietary fat intake on the expression of genes encoding proteins necessary for fatty acid transport and ß-oxidation in skeletal muscle. <br /><b>Design</b>: Fourteen well-trained male cyclists and triathletes with a mean (± SE) age of 26.9 ± 1.7 y, weight of 73.7 ± 1.7 kg, and peak oxygen uptake of 67.0 ± 1.3 mL ˙ kg-1 ˙ min-1 consumed either a high-fat diet (HFat: > 65% of energy as lipids) or an isoenergetic high-carbohydrate diet (HCho: 70–75% of energy as carbohydrate) for 5 d in a crossover design. On day 1 (baseline) and again after 5 d of dietary intervention, resting muscle and blood samples were taken. Muscle samples were analyzed for gene expression [fatty acid translocase (FAT/CD36), plasma membrane fatty acid binding protein (FABPpm), carnitine palmitoyltransferase I (CPT I), ß-hydroxyacyl-CoA dehydrogenase (ß-HAD), and uncoupling protein 3 (UCP3)] and concentrations of the proteins FAT/CD36 and FABPpm. <br /><b>Results</b>: The gene expression of FAT/CD36 and ß -HAD and the gene abundance of FAT/CD36 were greater after the HFat than after the HCho diet (P < 0.05). Messenger RNA expression of FABPpm, CPT I, and UCP-3 did not change significantly with either diet. <b><br />Conclusions</b>: A rapid and marked capacity for changes in dietary fatty acid availability to modulate the expression of mRNA-encoding proteins is necessary for fatty acid transport and oxidative metabolism. This finding is evidence of nutrient-gene interactions in human skeletal muscle.<br /> |
Identificador | |
Idioma(s) |
eng |
Publicador |
American Society for Clinical Nutrition Inc |
Relação |
http://dro.deakin.edu.au/eserv/DU:30002027/n20030373.pdf http://www.ajcn.org/cgi/content/full/77/2/313 |
Direitos |
2003, American Society for Clinical Nutrition |
Palavras-Chave | #Diet-gene interaction #messenger RNA #high-fat diet #skeletal muscle #metabolism #dietary intervention #fat oxidation #carbohydrate oxidation #gene expression |
Tipo |
Journal Article |