Development of a sequential injection chromatography (SIC) method for determination of simazine, atrazine, and propazine
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
This paper describes the development of a sequential injection chromatography (SIC) procedure for separation and quantification of the herbicides simazine, atrazine, and propazine exploring the low backpressure of a 2.5 cm long monolithic C(18) column. The separation of the three compounds was achieved in less than 90 s with resolution > 1.5 using a mobile phase composed by ACN/1.25 mmol/L acetate buffer (pH 4.5) at the volumetric ratio of 35:65 and flow rate of 40 mu L/s. Detection was made at 223 nm using a flow cell with 40 mm of optical path length. The LOD was 10 mu g/L for the three triazines and the quantification limits were of 30 mu g/L for simazine and propazine and 40 mu g/L for atrazine. The sampling frequency is 27 samples per hour, consuming 1.1 mL of ACN per analysis. The proposed methodology was applied to spiked water samples and no statistically significant differences were observed in comparison to a conventional HPLC-UV method. The major metabolites of atrazine and other herbicides did not interfere in the analysis, being eluted from the column either together with the unretained peak, or at retention times well-resolved from the studied compounds. Fundacao de Amparo & Pesquisa do Estado de Sao Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
Identificador |
JOURNAL OF SEPARATION SCIENCE, v.32, n.4, p.494-500, 2009 1615-9306 http://producao.usp.br/handle/BDPI/31230 10.1002/jssc.200800563 |
Idioma(s) |
eng |
Publicador |
WILEY-V C H VERLAG GMBH |
Relação |
Journal of Separation Science |
Direitos |
restrictedAccess Copyright WILEY-V C H VERLAG GMBH |
Palavras-Chave | #Environmental analysis #Separation methods #Triazines #PERFORMANCE LIQUID-CHROMATOGRAPHY #SOLID-PHASE EXTRACTION #MONOLITHIC STATIONARY PHASES #TRIAZINE HERBICIDES #MASS-SPECTROMETRY #AQUATIC ECOSYSTEMS #WATER SAMPLES #S-TRIAZINES #METABOLITES #SEPARATION #Chemistry, Analytical |
Tipo |
article original article publishedVersion |