Sequence and Structural Analysis of the 5 ` Noncoding Region of Hepatitis C Virus in Patients With Chronic Infection
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
Hepatitis C virus (HCV), exhibits considerable genetic diversity, but presents a relatively well conserved 5 ` noncoding region (5 ` NCR) among all genotypes. In this study, the structural features and translational efficiency of the HCV 5 ` NCR sequences were analyzed using the programs RNAfold, RNAshapes and RNApdist and with a bicistronic dual luciferase expression system, respectively. RNA structure prediction software indicated that base substitutions will alter potentially the 5 ` NCR structure. The heterogeneous sequence observed on 5 ` NCR led to important changes in their translation efficiency in different cell culture lines. Interactions of the viral RNA with cellular transacting factors may vary according to the cell type and viral genome polymorphisms that may result in the translational efficiency observed. J. Med. Virol. 81: 1212-1219, 2009. (C) 2009 Wiley-Liss, Inc. |
Identificador |
JOURNAL OF MEDICAL VIROLOGY, v.81, n.7, p.1212-1219, 2009 0146-6615 http://producao.usp.br/handle/BDPI/30392 10.1002/jmv.21507 |
Idioma(s) |
eng |
Publicador |
WILEY-LISS |
Relação |
Journal of Medical Virology |
Direitos |
restrictedAccess Copyright WILEY-LISS |
Palavras-Chave | #HCV #5 ` NCR #RNA translation #INTERNAL RIBOSOME-ENTRY #QUASI-SPECIES PRESENT #UNTRANSLATED REGION #SECONDARY STRUCTURE #TRANSLATION EFFICIENCIES #RNA #SITE #INITIATION #HCV #GENOME #Virology |
Tipo |
article original article publishedVersion |