The small nuclear ribonucleoprotein U1A interacts with NS5 from yellow fever virus
| Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
|---|---|
| Data(s) |
20/10/2012
20/10/2012
2011
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| Resumo |
The flavivirus NS5 protein is one of the most important proteins of the replication complex, and cellular proteins can interact with it. This study shows for the first time that the yellow fever virus (YFV) NS5 protein is able to interact with U1A, a protein involved in splicing and polyadenylation. We confirmed this interaction by GST-pulldown assay and by co-immunoprecipitation in YFV-infected cells. A region between amino acids 368 and 448 was identified as the site of interaction of the NS5 protein with U1A. This region was conserved among some flaviviruses of medical importance. The implications of this interaction for flavivirus replication are discussed. FAPESP[04/11098] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP[05/03260-7] CNPq[473613/2007-7] Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) CNPq[566289/2008-3] Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
| Identificador |
ARCHIVES OF VIROLOGY, v.156, n.6, p.931-938, 2011 0304-8608 http://producao.usp.br/handle/BDPI/30205 10.1007/s00705-011-0927-x |
| Idioma(s) |
eng |
| Publicador |
SPRINGER WIEN |
| Relação |
Archives of Virology |
| Direitos |
restrictedAccess Copyright SPRINGER WIEN |
| Palavras-Chave | #LA PROTEIN BINDS #DENGUE VIRUS #INFECTED-CELLS #POLY(A) TAIL #POLY(A)-BINDING PROTEIN #POLYADENYLATION SIGNAL #IMPORTIN ALPHA/BETA #MOSAIC POTEXVIRUS #RNA-POLYMERASE #3`-END REPAIR #Virology |
| Tipo |
article original article publishedVersion |
