Properties of lipophilic nucleoside monolayers at the air-water interface


Autoria(s): MONTANHA, E. A.; PAVINATTO, Felipe José; CASELI, L.; KACZMAREK, O.; LIEBSCHER, J.; HUSTER, D.; OLIVEIRA JUNIOR, Osvaldo Novais de
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2010

Resumo

The capability of self-assembly and molecular recognition of biomolecules is essential for many nanotechnological applications, as in the use of alkyl-modified nucleosides and oligonucleotides to increase the cellular uptake of DNA and RNA. In this study, we show that a lipophilic nucleoside, which is an isomer mixture of 2`-palmitoyluridin und 3`-palmitoyluridin, forms Langmuir monolayers and Langmuir-Blodgett films as a typical amphiphile, though with a smaller elasticity. The nucleoside may be incorporated into dipalmitoyl phosphatidyl choline (DPPC) monolayers that serve as a simplified cell membrane model. The molecular-level interactions between the nucleoside and DPPC led to a remarkable condensation of the mixed monolayer, which affected both surface pressure and surface potential isotherms. The morphology of the mixed monolayers was dominated by the small domains of the nucleoside. The mixed monolayers could be deposited onto solid substrates as a one-layer Langmuir Blodgett film that displayed UV-vis absorption spectra typical of aggregated nucleosides owing to the interaction between the nucleoside and DPPC. The formation of solid films with DNA building blocks in the polar heads may open the way for devices and sensors be produced to exploit their molecular recognition properties. (C) 2010 Elsevier B.V. All rights reserved.

FAPESP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

CNPq

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

CAPES

Bundesministeriums für Bildung und Forschung (BMBF)

Federal Ministry of Education and Research (BMBF)[0312027 C]

Federal Ministry of Education and Research (BMBF)[D 0312018]

Bundesministeriums für Bildung und Forschung (BMBF)

Identificador

COLLOIDS AND SURFACES B-BIOINTERFACES, v.77, n.2, p.161-165, 2010

0927-7765

http://producao.usp.br/handle/BDPI/29878

10.1016/j.colsurfb.2010.01.017

http://dx.doi.org/10.1016/j.colsurfb.2010.01.017

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

Colloids and Surfaces B-biointerfaces

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #Lipophilic nucleosides #Langmuir monolayers #Langmuir-Blodgett films #Model membrane #Nanotechnology #LANGMUIR-BLODGETT-FILMS #CELL-MEMBRANE MODELS #MOLECULAR RECOGNITION #PHOSPHOLIPID MONOLAYERS #LIPID-MEMBRANES #ASSEMBLIES #PROTEIN #ACID #OLIGONUCLEOTIDES #AMPHIPHILES #Biophysics #Chemistry, Physical #Materials Science, Biomaterials
Tipo

article

original article

publishedVersion