Properties of lipophilic nucleoside monolayers at the air-water interface
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2010
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Resumo |
The capability of self-assembly and molecular recognition of biomolecules is essential for many nanotechnological applications, as in the use of alkyl-modified nucleosides and oligonucleotides to increase the cellular uptake of DNA and RNA. In this study, we show that a lipophilic nucleoside, which is an isomer mixture of 2`-palmitoyluridin und 3`-palmitoyluridin, forms Langmuir monolayers and Langmuir-Blodgett films as a typical amphiphile, though with a smaller elasticity. The nucleoside may be incorporated into dipalmitoyl phosphatidyl choline (DPPC) monolayers that serve as a simplified cell membrane model. The molecular-level interactions between the nucleoside and DPPC led to a remarkable condensation of the mixed monolayer, which affected both surface pressure and surface potential isotherms. The morphology of the mixed monolayers was dominated by the small domains of the nucleoside. The mixed monolayers could be deposited onto solid substrates as a one-layer Langmuir Blodgett film that displayed UV-vis absorption spectra typical of aggregated nucleosides owing to the interaction between the nucleoside and DPPC. The formation of solid films with DNA building blocks in the polar heads may open the way for devices and sensors be produced to exploit their molecular recognition properties. (C) 2010 Elsevier B.V. All rights reserved. FAPESP Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) CNPq Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) CAPES Bundesministeriums für Bildung und Forschung (BMBF) Federal Ministry of Education and Research (BMBF)[0312027 C] Federal Ministry of Education and Research (BMBF)[D 0312018] Bundesministeriums für Bildung und Forschung (BMBF) |
Identificador |
COLLOIDS AND SURFACES B-BIOINTERFACES, v.77, n.2, p.161-165, 2010 0927-7765 http://producao.usp.br/handle/BDPI/29878 10.1016/j.colsurfb.2010.01.017 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE BV |
Relação |
Colloids and Surfaces B-biointerfaces |
Direitos |
restrictedAccess Copyright ELSEVIER SCIENCE BV |
Palavras-Chave | #Lipophilic nucleosides #Langmuir monolayers #Langmuir-Blodgett films #Model membrane #Nanotechnology #LANGMUIR-BLODGETT-FILMS #CELL-MEMBRANE MODELS #MOLECULAR RECOGNITION #PHOSPHOLIPID MONOLAYERS #LIPID-MEMBRANES #ASSEMBLIES #PROTEIN #ACID #OLIGONUCLEOTIDES #AMPHIPHILES #Biophysics #Chemistry, Physical #Materials Science, Biomaterials |
Tipo |
article original article publishedVersion |