Study of DNA damage with a new system for irradiation of samples in a nuclear reactor

In this paper, we report results of a quantitative analysis of the effects of neutrons on DNA, and, specifically, the production of simple and double breaks of plasmid DNA in aqueous solutions with different concentrations of free-radical scavengers. The radiation damage to DNA was evaluated by electrophoresis through agarose gels. The neutron and gamma doses were measured separately with thermoluminescent detectors. In this work, we have also demonstrated usefulness of a new system for positioning and removing samples in channel BH#3 of the IEA-R1 reactor at the Instituto de Pesquisas Energeticas e Nucleares (Brazil) without necessity of interrupting the reactor operation. (C) 2010 Elsevier Ltd. All rights reserved.

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, FAPESP[097555-0]

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Latin-American Centre of Physics (CLAF)

Latin-American Centre for Physics (CLAF)

International Centre for Theoretical Physics (ICTP) of Trieste, Italy

International Centre for Theoretical Physics (ICTP) of Trieste, Italy