Trypanosoma cruzi in Brazilian Amazonia: Lineages TCI and TCIIa in wild primates, Rhodnius spp. and in humans with Chagas disease associated with oral transmission
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
In this study, we provide phylogenetic and biogeographic evidence that the Trypanosomo cruzi lineages T. cruzi I (TCI) and T. cruzi IIa (TCIIa) circulate amongst non-human primates in Brazilian Amazonia, and are transmitted by Rhodnius species in overlapping arboreal transmission cycles, sporadically infecting humans. TO presented higher prevalence rates, and no lineages other than TCI and TCIIa were found in this study in wild monkeys and Rhodnius from the Amazonian region. We characterised TO and TCIIa from wild primates (16 TO and five TCIIa), Rhodnius spp, (13 TCI and nine TCIIa), and humans with Chagas disease associated with oral transmission (14 TO and five TCIIa) in Brazilian Amazonia. To our knowledge, TCIIa had not been associated with wild monkeys until now. Polymorphisms of ssrDNA, cytochrome b gene sequences and randomly amplified polymorphic DNA (RAPD) patterns clearly separated TCIIa from TCIIb-e and TCI lineages, and disclosed small intra-lineage polymorphisms amongst isolates from Amazonia. These data are important in understanding the complexity of the transmission cycles, genetic structure, and evolutionary history of T cruzi populations circulating in Amazonia, and they contribute to both the unravelling of human infection routes and the pathological peculiarities of Chagas disease in this region. (C) 2008 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Brazilian agencies CNPq FAPESP (PRONEX) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) IEC IEC ECLAT ECLAT Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) CAPES (PRODOC-PROTAX) |
Identificador |
INTERNATIONAL JOURNAL FOR PARASITOLOGY, v.39, n.5, p.615-623, 2009 0020-7519 http://producao.usp.br/handle/BDPI/28545 10.1016/j.ijpara.2008.09.015 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCI LTD |
Relação |
International Journal for Parasitology |
Direitos |
restrictedAccess Copyright ELSEVIER SCI LTD |
Palavras-Chave | #Trypanosoma cruzi lineages TCI and TCIIa #Chagas disease #Oral infection #Non-human primates #Amazonia #ssrDNA #Cytochrome b #Evolution #Phylogeny #AMPLIFIED POLYMORPHIC DNA #SUBUNIT RIBOSOMAL-RNA #MINI-EXON GENE #MOLECULAR EPIDEMIOLOGY #LEONTOPITHECUS-ROSALIA #PHYLOGENETIC LINEAGES #SYLVATIC MAMMALS #TRIATOMINE BUGS #INFECTION-RATES #UNITED-STATES #Parasitology |
Tipo |
article original article publishedVersion |