Prefrontal afferents to the dorsal raphe nucleus in the rat


Autoria(s): GONCALVES, Luciano; NOGUEIRA, Maria Ines; SHAMMAH-LAGNADO, Sara Joyce; METZGER, Martin
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

The prefrontal cortex (PFC) receives strong inputs from monoaminergic cell groups in the brainstem and also sends projections to these nuclei. Recent evidence suggests that the PFC exerts a powerful top-down control over the dorsal raphe nucleus (DR) and that it may be involved in the actions of pharmaceutical drugs and drugs of abuse. In the light of these findings, the precise origin of prefrontal inputs to DR was presently investigated by using the cholera toxin subunit b (CTb) as retrograde tracer. All the injections placed in DR produced retrograde labeling in the medial, orbital, and lateral divisions of the PFC as well as in the medial part of the frontal polar cortex. The labeling was primarily located in layer V. Remarkably, labeling in the medial PFC was denser in its ventral part (infralimbic and ventral prelimbic cortices) than in its dorsal part (dorsal prelimbic, anterior cingulate and medial precentral cortices). After injections in the rostral or caudal DR, the largest number of labeled neurons was observed in the medial PFC, whereas after injections in the mid-rostrocaudal DR, the labeled neurons were more homogeneously distributed in the three main PFC divisions. A cluster of labeled neurons also was observed around the apex of the rostral pole of the accumbens, especially after rostral and mid-rostrocaudal DR injections. Overall, these results confirm the existence of robust preftontal projections to DR, mainly derived from the ventral part of the medial PFC, and underscore a substantial contribution of the frontal polar cortex. (C) 2008 Elsevier Inc. All rights reserved.

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

FAPESP[2004/06790-4]

FAPESP[2005/51607-6]

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

FAPESP[2006/53068-8]

Identificador

BRAIN RESEARCH BULLETIN, v.78, n.4/Mai, p.240-247, 2009

0361-9230

http://producao.usp.br/handle/BDPI/28467

10.1016/j.brainresbull.2008.11.012

http://dx.doi.org/10.1016/j.brainresbull.2008.11.012

Idioma(s)

eng

Publicador

PERGAMON-ELSEVIER SCIENCE LTD

Relação

Brain Research Bulletin

Direitos

restrictedAccess

Copyright PERGAMON-ELSEVIER SCIENCE LTD

Palavras-Chave #Serotonin #Prefrontal cortex #Raphe nuclei #Top-down control #Limbic #Retrograde tracing #PHASEOLUS-VULGARIS-LEUKOAGGLUTININ #MEDIODORSAL THALAMIC NUCLEUS #VENTRAL TEGMENTAL AREA #CORTICAL PROJECTIONS #5-HT2A RECEPTORS #STRESSOR CONTROLLABILITY #SEROTONIN(2A) RECEPTORS #ASCENDING PROJECTIONS #BEHAVIORAL-CONTROL #GABAERGIC NEURONS #Neurosciences
Tipo

article

original article

publishedVersion