PHA(MCL) biosynthesis systems in Pseudomonas aeruginosa and Pseudomonas putida strains show differences on monomer specificities
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
The production of PHA from plant oils by Pseudomonas species soil isolated from a sugarcane crop was evaluated. Out of 22 bacterial strains three were able to use efficiently plant oils to grow and to accumulate PHA. Pseudomonas putida and Pseudomonas aeruginosa strains produced PHA presenting differences on monomer composition compatible with variability on monomer specificity of their PHA biosynthesis system. The molar fraction of 3-hydroxydodecanoate detected in the PHA was linearly correlated to the oleic acid supplied. A non-linear relationship between the molar fractions of 3-hydroxy-6-dodecenoate (3HDd Delta(6)) detected in PHA and the linoleic acid supplied was observed, compatible with saturation in the biosynthesis system capability to channel intermediate of P-oxidation to PHA synthesis. Although P. putida showed a higher 3HDd Delta(6) yield from linoleic acid when compared to P. aeruginosa, in both species it was less than 10% of the maximum theoretical value. These results contribute to the knowledge about the biosynthesis of PHA with a controlled composition from plant oils allowing in the future establishing the production of these polyesters as tailor-made polymers. (C) 2009 Elsevier B.V. All rights reserved. FAPESP[03/01602-2] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP[00/00689-9] FAPESP[03/11795-2] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) CNPq (PADCT) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) |
Identificador |
JOURNAL OF BIOTECHNOLOGY, v.143, n.2, p.111-118, 2009 0168-1656 http://producao.usp.br/handle/BDPI/28450 10.1016/j.jbiotec.2009.06.014 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE BV |
Relação |
Journal of Biotechnology |
Direitos |
restrictedAccess Copyright ELSEVIER SCIENCE BV |
Palavras-Chave | #Polyhydroxyalkanoic acid #Pseudomonas #Plant oils #Fatty acids #Tailor-made polymers #MEDIUM-CHAIN-LENGTH #BACTERIAL POLYHYDROXYALKANOIC ACIDS #FREE FATTY-ACIDS #ESCHERICHIA-COLI #FLUORESCENT PSEUDOMONADS #VEGETABLE-OILS #PHA PRODUCTION #CARBON-SOURCES #MCL-PHAS #OLEOVORANS #Biotechnology & Applied Microbiology |
Tipo |
article original article publishedVersion |