Effects of gamma-radiation on the fungus Alternaria alternata in artificially inoculated cereal samples
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2009
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Resumo |
The objective of this study was to evaluate the effects of different gamma-radiation doses on the growth of Alternaria alternata in artificially inoculated cereal samples. Seeds and grains were divided into four groups: Control Group (not irradiated), and Groups 1, 2 and 3, inoculated with an A. alternata spore suspension (1 x 10(6) spores/mL) and exposed to 2, 5 and 10 kGy, respectively. Serial dilutions of the samples were prepared and seeded on DRBC (dichloran rose bengal chloramphenicol agar) and DCMA (dichloran chloramphenicol malt extract agar) media, after which the number of colony-forming units per gram was determined in each group. In addition, fungal morphology after irradiation was analyzed by scanning electron microscopy (SEM). The results showed that ionizing radiation at a dose of 5 kGy was effective in reducing the growth of A. alternata. However, a dose of 10 kGy was necessary to inhibit fungal growth completely. SEM made it possible to visualize structural alterations induced by the different gamma-radiation doses used. (C) 2009 Elsevier Ltd. All rights reserved. |
Identificador |
APPLIED RADIATION AND ISOTOPES, v.67, n.9, p.1622-1628, 2009 0969-8043 http://producao.usp.br/handle/BDPI/28446 10.1016/j.apradiso.2009.03.004 |
Idioma(s) |
eng |
Publicador |
PERGAMON-ELSEVIER SCIENCE LTD |
Relação |
Applied Radiation and Isotopes |
Direitos |
restrictedAccess Copyright PERGAMON-ELSEVIER SCIENCE LTD |
Palavras-Chave | #Foods #Cereals #Alternaria alternata #gamma-radiation #Fungi #WEATHER-DAMAGED WHEAT #WATER ACTIVITY #FUSARIUM-MONILIFORME #ASPERGILLUS-FLAVUS #MYCOTOXINS #MYCOFLORA #MAIZE #IRRADIATION #HARVEST #GRAINS #Chemistry, Inorganic & Nuclear #Nuclear Science & Technology #Radiology, Nuclear Medicine & Medical Imaging |
Tipo |
article original article publishedVersion |