Exploring Bacterial Diversity of Endodontic Microbiota by Cloning and Sequencing 16S rRNA
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2011
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Resumo |
Introduction: The characterization of microbial communities infecting the endodontic system in each clinical condition may help on the establishment of a correct prognosis and distinct strategies of treatment. The purpose of this study was to determine the bacterial diversity in primary endodontic infections by 16S ribosomal-RNA (rRNA) sequence analysis. Methods: Samples from root canals of untreated asymptomatic teeth (n = 12) exhibiting periapical lesions were obtained, 165 rRNA bacterial genomic libraries were constructed and sequenced, and bacterial diversity was estimated. Results: A total of 489 clones were analyzed (mean, 40.7 +/- 8.0 clones per sample). Seventy phylotypes were identified of which six were novel phylotypes belonging to the family Ruminococcaceae. The mean number of taxa per canal was 10.0, ranging from 3 to 21 per sample; 65.7% of the cloned sequences represented phylotypes for which no cultivated isolates have been reported. The most prevalent taxa were Atopobium rimae (50.0%), Dialister invisus, Pre-votella oris, Pseudoramibacter alactolyticus, and Tannerella forsythia (33.3%). Conclusions: Although several key species predominate in endodontic samples of asymptomatic cases with periapical lesions, the primary endodontic infection is characterized by a wide bacterial diversity, which is mostly represented by members of the phylum Firmicutes belonging to the class Clostridia followed by the phylum Bacteroidetes. (J Ended 2011;37:922-926) Sao Paulo Research Foundation (FAPESP)[07/52492-3] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Sao Paulo Research Foundation (FAPESP)[06/59856-8] Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) |
Identificador |
JOURNAL OF ENDODONTICS, v.37, n.7, p.922-926, 2011 0099-2399 http://producao.usp.br/handle/BDPI/28343 10.1016/j.joen.2011.04.007 |
Idioma(s) |
eng |
Publicador |
ELSEVIER SCIENCE INC |
Relação |
Journal of Endodontics |
Direitos |
restrictedAccess Copyright ELSEVIER SCIENCE INC |
Palavras-Chave | #165 rRNA #bacterial diversity #clinical study #in vivo #metagenomic analysis #primary endodontic infection #MOLECULAR ANALYSIS #ROOT CANALS #ATOPOBIUM-RIMAE #CLONAL ANALYSIS #INFECTIONS #IDENTIFICATION #MICROORGANISMS #FLORA #PERIODONTITIS #PHYLOTYPES #Dentistry, Oral Surgery & Medicine |
Tipo |
article original article publishedVersion |