Putative antinociceptive action of nitric oxide in the caudal part of the spinal trigeminal nucleus during chronic carrageenan-induced arthritis in the rat temporomandibular joint


Autoria(s): TESSER-VISCAINO, Silvia A.; DENADAI-SOUZA, Alexandre; TEIXEIRA, Simone A.; ERVOLINO, Edilson; CRUZ-RIZZOLO, Roelf J.; COSTA, Soraia K.; MUSCARA, Marcelo N.; CASATTI, Claudio A.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

In order to investigate a putative role for nitric oxide (NO) in the central nociceptive processing following carrageenan-induced arthritis in the rat temporomandibular joint (TMJ), we analyzed the immunoreactivity, gene expression and activity of nitric oxide synthases (NOS) in the caudal part of the spinal trigeminal nucleus (Sp5C) during the acute (24 h), chronic (15 days) and chronic-active (14 days-24 h) arthritis. In addition, evaluation of head-withdrawal threshold was carried out in all phases of arthritis under chronic inhibition of nNOS with the selective inhibitor 7-nitroindazole (7-NI). Neurons with nNOS-like immunoreactivity (nNOS-LI) were concentrated mainly in the lamina II of the Sp5C, showing no significant statistical difference during arthritis. Only a discrete percentage of nNOS-LI neurons expressed Fos immunoreactivity. The mRNA expression for both nNOS and endothelial nitric oxide synthases (eNOS) presented no noticeable differences among the groups. No expression of inducible nitric oxide synthase (iNOS) was detected in the Sp5C by either immunohistochemistry or reverse-transcription polymerase chain reaction (RTPCR). Ca(2+)-dependent NOS activity in the ipsilateral Sp5C was significantly higher (108.3 +/- 49.2%; P<0.01) in animals during the chronic arthritis. Interestingly, this increased activity was completely abolished 24 h later, in the chronic-active arthritis. Finally, head-withdrawal threshold decreased significantly in the chronic arthritis in animals under 7-NI chronic inhibition. In conclusion, nNOS immunoreactivity and mRNA expression are stable in the Sp5C during TMJ arthritis evolution, but its activity significantly increases in the chronic-phases supporting an antinociceptive role of the nNOS as evidenced by pain threshold experiment. (C) 2009 Elsevier B.V. All rights reserved.

Identificador

BRAIN RESEARCH, v.1302, p.85-96, 2009

0006-8993

http://producao.usp.br/handle/BDPI/28182

10.1016/j.brainres.2009.09.056

http://dx.doi.org/10.1016/j.brainres.2009.09.056

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

Brain Research

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #Arthritis #Nitric oxide synthase #Spinal trigeminal nucleus #Temporomandibular joint #DEPENDENT PROTEIN-KINASE #MEDULLARY DORSAL-HORN #BRAIN-STEM MECHANISMS #NO-CGMP PATHWAY #OROFACIAL PAIN #PERSISTENT PAIN #FOS PROTEIN #SYNTHASE IMMUNOREACTIVITY #INFLAMMATORY HYPERALGESIA #CENTRAL SENSITIZATION #Neurosciences
Tipo

article

original article

publishedVersion