The Ventral Premammillary Nucleus Links Fasting-Induced Changes in Leptin Levels and Coordinated Luteinizing Hormone Secretion


Autoria(s): DONATO JR., Jose; Silva, Renata Juliana da; SITA, Luciane V.; LEE, Syann; LEE, Charlotte; LACCHINI, Silvia; BITTENCOURT, Jackson C.; FRANCI, Celso R.; CANTERAS, Newton S.; ELIAS, Carol F.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2009

Resumo

Physiological conditions of low leptin levels like those observed during negative energy balance are usually characterized by the suppression of luteinizing hormone (LH) secretion and fertility. Leptin administration restores LH levels and reproductive function. Leptin action on LH secretion is thought to be mediated by the brain. However, the neuronal population that mediates this effect is still undefined. The hypothalamic ventral premammillary nucleus (PMV) neurons express a dense concentration of leptin receptors and project to brain areas related to reproductive control. Therefore, we hypothesized that the PMV is well located to mediate leptin action on LH secretion. To test our hypothesis, we performed bilateral excitotoxic lesions of the PMV in adult female rats. PMV-lesioned animals displayed a clear disruption of the estrous cycle, remaining in anestrus for 15-20 d. After apparent recovery of cyclicity, animals perfused in the afternoon of proestrus showed decreased Fos immunoreactivity in the anteroventral periventricular nucleus and in gonadotropin releasing hormone neurons. PMV-lesioned animals also displayed decreased estrogen and LH secretion on proestrus. Lesions caused no changes in mean food intake and body weight up to 7 weeks after surgery. We further tested the ability of leptin to induce LH secretion in PMV-lesioned fasted animals. We found that complete lesions of the PMV precluded leptin stimulation of LH secretion on fasting. Our findings demonstrate that the PMV is a key site linking changing levels of leptin and coordinated control of reproduction.

University of Texas Southwestern Medical Center (UTSW)

University of Texas Southwestern Medical Center (UTSW)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

CNPq Conselho Nacional de Desenvolvimento Cientifico e Tecnologico

FAPESP Fundacao de Amparo a Pesquisa do Estado de Sao Paulo[04/13850-30]

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

FAPESP Fundacao de Amparo a Pesquisa do Estado de Sao Paulo[04/13849-5]

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

FAPESP Fundacao de Amparo a Pesquisa do Estado de Sao Paulo[04/09638-9]

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

FAPESP Fundacao de Amparo a Pesquisa do Estado de Sao Paulo[05/58997-4]

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

FAPESP Fundacao de Amparo a Pesquisa do Estado de Sao Paulo[05/59286-4]

National Institutes of Health (NIH)[1PL1DK081182-01]

U.S. National Institutes of Health (NIH)

Identificador

JOURNAL OF NEUROSCIENCE, v.29, n.16, p.5240-5250, 2009

0270-6474

http://producao.usp.br/handle/BDPI/27867

10.1523/JNEUROSCI.0405-09.2009

http://dx.doi.org/10.1523/JNEUROSCI.0405-09.2009

Idioma(s)

eng

Publicador

SOC NEUROSCIENCE

Relação

Journal of Neuroscience

Direitos

restrictedAccess

Copyright SOC NEUROSCIENCE

Palavras-Chave #GONADOTROPIN-RELEASING-HORMONE #ANTEROVENTRAL PERIVENTRICULAR NUCLEUS #ESTROGEN POSITIVE FEEDBACK #FEMALE RATS #NEUROPEPTIDE-Y #ENERGY-BALANCE #HYPOTHALAMIC EXPRESSION #GLUCOSE-HOMEOSTASIS #RECOMBINANT LEPTIN #ARCUATE NUCLEUS #Neurosciences
Tipo

article

original article

publishedVersion