Increased Expression of GluR2-flip in the Hippocampus of the Wistar Audiogenic Rat Strain After Acute and Kindled Seizures


Autoria(s): GITAI, Daniel Leite Goes; MARTINELLI, Heloisa Nakashima; VALENTE, Valeria; PEREIRA, Marilia G. Alves Goulart; OLIVEIRA, Jose Antonio Cortes; ELIAS, Carol Fuzeti; BITTENCOURT, Jackson Cioni; LEITE, Joao Pereira; COSTA-NETO, Claudio Miguel; GARCIA-CAIRASCO, Norberto; PACO-LARSON, Maria Luisa
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2010

Resumo

The Wistar Audiogenic Rat (WAR) is an epileptic-prone strain developed by genetic selection from a Wistar progenitor based on the pattern of behavioral response to sound stimulation. Chronic acoustic stimulation protocols of WARs (audiogenic kindling) generate limbic epileptogenesis, confirmed by ictal semiology, amygdale, and hippocampal EEG, accompanied by hippocampal and amygdala cell loss, as well as neurogenesis in the dentate gyrus (DG). In an effort to identify genes involved in molecular mechanisms underlying epileptic process, we used suppression-subtractive hybridization to construct normalized cDNA library enriched for transcripts expressed in the hippocampus of WARs. The most represented gene among the 133 clones sequenced was the ionotropic glutamate receptor subunit II (GluR2), a member of the a-amino-3-hydroxy-5-methyl-4-isoxazoleopropionic acid (AMPA) receptor. Although semiquantitative RT-PCR analysis shows that the hippocampal levels of the GluR2 subunits do not differ between naive WARs and their Wistar counterparts, we observed that the expression of the transcript encoding the splice-variant GluR2-flip is increased in the hippocampus of WARs submitted to both acute and kindled audiogenic seizures. Moreover, using in situ hybridization, we verified upregulation of GluR2-flip mainly in the CA1 region, among the hippocampal subfields of audiogenic kindled WARs. Our findings on differential upregulation of GluR2-flip isoform in the hippocampus of WARs displaying audiogenic seizures is original and agree with and extend previous immunohistochemical for GluR2 data obtained in the Chinese P77PMC audiogenic rat strain, reinforcing the association of limbic AMPA alterations with epileptic seizures. (C) 2009 Wiley-Liss, Inc.

Identificador

HIPPOCAMPUS, v.20, n.1, p.125-133, 2010

1050-9631

http://producao.usp.br/handle/BDPI/27853

10.1002/hipo.20590

http://dx.doi.org/10.1002/hipo.20590

Idioma(s)

eng

Publicador

WILEY-LISS

Relação

Hippocampus

Direitos

restrictedAccess

Copyright WILEY-LISS

Palavras-Chave #AMPA receptor #audiogenic seizure #audiogenic kindling #gene expression #EPILEPSY-PRONE RATS #KAINATE-INDUCED EPILEPSY #AMPA RECEPTOR SUBUNITS #INFERIOR COLLICULUS #GENE-EXPRESSION #EPILEPTIFORM ACTIVITY #LIMBIC STRUCTURES #TRANSGENIC MICE #WAR STRAIN #C-FOS #Neurosciences
Tipo

article

original article

publishedVersion