Protein identification pipeline for the homology-driven proteomics


Autoria(s): JUNQUEIRA, Magno; SPIRIN, Victor; BALBUENA, Tiago Santana; THOMAS, Henrik; ADZHUBEI, Ivan; SUNYAEV, Shamil; SHEVCHENKO, Andrej
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2008

Resumo

Homology-driven proteomics is a major tool to characterize proteomes of organisms with unsequenced genomes. This paper addresses practical aspects of automated homology-driven protein identifications by LC-MS/MS on a hybrid LTQ orbitrap mass spectrometer. All essential software elements supporting the presented pipeline are either hosted at the publicly accessible web server, or are available for free download. (C) 2008 Elsevier B.V. All rights reserved.

U.S. National Institutes of Health (NIH)

NIH NIGMS[1R01GM070986-01A1]

Identificador

JOURNAL OF PROTEOMICS, v.71, n.3, Special Issue, p.346-356, 2008

1874-3919

http://producao.usp.br/handle/BDPI/27694

10.1016/j.jprot.2008.07.003

http://dx.doi.org/10.1016/j.jprot.2008.07.003

Idioma(s)

eng

Publicador

ELSEVIER SCIENCE BV

Relação

Journal of Proteomics

Direitos

restrictedAccess

Copyright ELSEVIER SCIENCE BV

Palavras-Chave #MS/MS #De novo sequencing #MS BLAST #Sequence-similarity searches #Insect proteomics #Plant proteomics #MASS-SPECTROMETRY #SEQUENCE DATABASES #LC-MS/MS #PEPTIDE #VALIDATION #ORGANISMS #SEARCHES #DATASETS #GENOMES #GELS #Biochemical Research Methods
Tipo

article

original article

publishedVersion