Mitochondrial DNA Diversity of Orchid Bee Euglossa fimbriata (Hymenoptera: Apidae) Populations Assessed by PCR-RFLP
Contribuinte(s) |
UNIVERSIDADE DE SÃO PAULO |
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Data(s) |
20/10/2012
20/10/2012
2010
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Resumo |
Euglossa fimbriata is a euglossine species widely distributed in Brazil and occurring primarily in Atlantic Forest remnants. In this study, the genetic mitochondrial structure of E. fimbriata from six Atlantic Forest fragments was studied by RFLP analysis of three PCR-amplified mtDNA gene segments (16S, COI-COII, and cyt b). Ten composite haplotypes were identified, six of which were exclusive and represented singleton mitotypes. Low haplotype diversity (0.085-0.289) and nucleotide diversity (0.000-0.002) were detected within samples. AMOVA partitioned 91.13% of the overall genetic variation within samples and 8.87% (I center dot(st) = 0.089; P < 0.05) among samples. Pairwise comparisons indicated high levels of differentiation among some pairs of samples (I center dot(st) = 0.161-0.218; P < 0.05). These high levels indicate that these populations of E. fimbriata, despite their highly fragmented landscape, apparently have not suffered loss of genetic variation, suggesting that this particular population is not currently endangered. FBPN FBPN Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) PIBIC-CNPq |
Identificador |
BIOCHEMICAL GENETICS, v.48, n.3/Abr, p.326-341, 2010 0006-2928 http://producao.usp.br/handle/BDPI/27512 10.1007/s10528-009-9325-4 |
Idioma(s) |
eng |
Publicador |
SPRINGER/PLENUM PUBLISHERS |
Relação |
Biochemical Genetics |
Direitos |
restrictedAccess Copyright SPRINGER/PLENUM PUBLISHERS |
Palavras-Chave | #Euglossini #Euglossine bees #Genetic structure #mtDNA #PCR-RFLP #AMAZONIAN FOREST FRAGMENTS #BRAZILIAN ATLANTIC FOREST #GENETIC DIVERSITY #POLLINATOR DECLINE #MOLECULAR MARKERS #PHYLOGENY #CONSERVATION #BIOLOGY #MELIPONINI #DISTANCE #Biochemistry & Molecular Biology #Genetics & Heredity |
Tipo |
article original article publishedVersion |