Effects of N supply on the accumulation of photosynthetic pigments and photoprotectors in Gracilaria tenuistipitata (Rhodophyta) cultured under UV radiation


Autoria(s): BARUFI, Jose Bonomi; KORBEE, Nathalie; OLIVEIRA, Mariana C.; FIGUEROA, Felix L.
Contribuinte(s)

UNIVERSIDADE DE SÃO PAULO

Data(s)

20/10/2012

20/10/2012

2011

Resumo

We have studied the effects of nitrate supply under photosynthetic active radiation (PAR) plus ultraviolet radiation (UVR) exposure on photosynthetic pigments (chlorophyll a and carotenoids), photoprotective UV screen mycosporine-like amino acids (MAAs), and photosynthetic parameters, including the maximum quantum yield (F(v)/F(m)) and electron transport rate (ETR) on the red agarophyte Gracilaria tenuistipitata. Apical tips of G. tenuistipitata were cultivated under ten different concentrations of NO(3)(-) for 7 days. It has been shown that G. tenuistipitata cultured under laboratory conditions has the ability to accumulate high amounts of MAAs following a nitrate concentration-dependent manner under PAR+UVR. Two MAAs were identified, shinorine and porphyra-334. The relative concentration of the first increased under high concentrations of nitrate, while the second one decreased. The presence of antheraxanthin is reported for the first time in this macro-algae, which also contains zeaxanthin, lutein, and beta-carotene. The accumulation of pigments, photoprotective compounds, and photosynthetic parameters of G. tenuistipitata is directly related to N availability. All variables decreased under low N supplies and reached constant maximum values with supplements higher than 0.5 mM NO(3)(-). Our results suggest a high potential to acclimation and photoprotection against stress factors (including high PAR and UVR) directly related to N availability for G. tenuistipitata.

Ministry of Science and Innovation of Spain[CGL2008 05407-C03-01]

Junta de Andalucia research group[RNM-295]

National Council for Scientific and Technological Development (CNPq)[470417/2006-4]

National Council for Scientific and Technological Development (CNPq)[301217/2007-6]

Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)

Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) from the Ministry of Education in Brazil

Identificador

JOURNAL OF APPLIED PHYCOLOGY, v.23, n.3, Special Issue, p.457-466, 2011

0921-8971

http://producao.usp.br/handle/BDPI/27237

10.1007/s10811-010-9603-x

http://dx.doi.org/10.1007/s10811-010-9603-x

Idioma(s)

eng

Publicador

SPRINGER

Relação

Journal of Applied Phycology

Direitos

restrictedAccess

Copyright SPRINGER

Palavras-Chave #Carotenoids #Gracilaria tenuistipitata #In vivo chlorophyll a fluorescence #N availability #Photoprotection #Photosynthesis #THERMAL-ENERGY DISSIPATION #MARINE RED ALGAE #AMINO-ACIDS MAAS #ULTRAVIOLET-RADIATION #CAROTENOID COMPOSITION #XANTHOPHYLL-CYCLE #VAR. LIUI #CHLOROPHYLL FLUORESCENCE #NITROGEN AVAILABILITY #OXYGEN EVOLUTION #Biotechnology & Applied Microbiology #Marine & Freshwater Biology
Tipo

article

original article

publishedVersion