Synthesis of prebiotic galactooligosaccharides from lactose using bifidobacterial β-galactosidase (BbgIV) immobilised on DEAE-Cellulose, Q-Sepharose and amino-ethyl agarose
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15/01/2014
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Resumo |
The bifidobacterial β-galactosidase BbgIV was immobilised on DEAE-Cellulose and Q-Sepharose via ionic binding and on amino-ethyl- and glyoxal-agarose via covalent attachment, and was then used to catalyse the synthesis of galactooligosaccharides (GOS). The immobilisation yield exceeded 90 % using ionic binding, while it was low using aminoethyl agarose (25 – 28 %) and very low using glyoxal agarose (< 3 %). This was due to the mild conditions and absence of chemical reagents in ionic binding, compared to covalent attachment. The maximum GOS yield obtained using DEAE-Cellulose and Q-Sepharose was similar to that obtained using free BbgIV (49 – 53 %), indicating the absence of diffusion limitation and mass transfer issues. For amino-ethyl agarose, however, the GOS yield obtained was lower (42 – 44 %) compared to that obtained using free BbgIV. All the supports tried significantly (P < 0.05) increased the BbgIV operational stability and the GOS synthesis productivity up to 55 °C. Besides, six successive GOS synthesis batches were performed using BbgIV immobilised on Q-Sepharose; all resulted in similar GOS yields, indicating the possibility of developing a robust synthesis process. Overall, the GOS synthesis operation performance using BbgIV was improved by immobilising the enzyme onto solid supports, in particular on Q-Sepharose |
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Identificador |
http://centaur.reading.ac.uk/35714/8/1-s2.0-S1369703X13003355-main.pdf http://centaur.reading.ac.uk/35714/1/immobilisation.pdf Osman, A., Symeou, S., Trisse, V., Watson, K. A. <http://centaur.reading.ac.uk/view/creators/90000363.html>, Tzortzis, G. and Charalampopoulos, D. <http://centaur.reading.ac.uk/view/creators/90000471.html> (2014) Synthesis of prebiotic galactooligosaccharides from lactose using bifidobacterial β-galactosidase (BbgIV) immobilised on DEAE-Cellulose, Q-Sepharose and amino-ethyl agarose. Biochemical Engineering Journal, 82. pp. 188-199. ISSN 1369-703X doi: 10.1016/j.bej.2013.11.020 <http://dx.doi.org/10.1016/j.bej.2013.11.020> |
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en en |
Publicador |
Elsevier |
Relação |
http://centaur.reading.ac.uk/35714/ creatorInternal Watson, Kim A creatorInternal Charalampopoulos, Dimitris http://dx.doi.org/10.1016/j.bej.2013.11.020 10.1016/j.bej.2013.11.020 |
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Article PeerReviewed |