Riluzole neuroprotection in a parkinson’s disease model involves suppression of reactive astrocytosis but not GLT-1 regulation


Autoria(s): Carbone, Marica; Duty, Susan; Rattray, Marcus
Data(s)

2012

Resumo

Background Riluzole is a neuroprotective drug used in the treatment of motor neurone disease. Recent evidence suggests that riluzole can up-regulate the expression and activity of the astrocyte glutamate transporter, GLT-1. Given that regulation of glutamate transport is predicted to be neuroprotective in Parkinson's disease, we tested the effect of riluzole in parkinsonian rats which had received a unilateral 6-hydroxydopamine injection into the median forebrain bundle. Results Rats were treated with intraperitoneal riluzole (4 mg/kg or 8 mg/kg), 1 hour before the lesion then once daily for seven days. Riluzole produced a modest but significant attenuation of dopamine neurone degeneration, assessed by suppression of amphetamine-induced rotations, preservation of tyrosine hydroxylase positive neuronal cell bodies in the substantia nigra pars compacta and attenuation of striatal tyrosine hydroxylase protein loss. Seven days after 6-hydroxydopamine lesion, reactive astrocytosis was observed in the striatum, as determined by increases in expression of glial fibrillary acidic protein, however the glutamate transporter, GLT-1, which is also expressed in astrocytes was not regulated by the lesion. Conclusions The results confirm that riluzole is a neuroprotective agent in a rodent model of parkinson’s disease. Riluzole administration did not regulate GLT-1 levels but significantly reduced GFAP levels, in the lesioned striatum. Riluzole suppression of reactive astrocytosis is an intriguing finding which might contribute to the neuroprotective effects of this drug.

Formato

text

Identificador

http://centaur.reading.ac.uk/27343/2/1471-2202-13-38.pdf

Carbone, M., Duty, S. and Rattray, M. <http://centaur.reading.ac.uk/view/creators/90000721.html> (2012) Riluzole neuroprotection in a parkinson’s disease model involves suppression of reactive astrocytosis but not GLT-1 regulation. BMC Neuroscience, 13 (1). 38. ISSN 1471-2202 doi: 10.1186/1471-2202-13-38 <http://dx.doi.org/10.1186/1471-2202-13-38>

Idioma(s)

en

Publicador

BioMed Central

Relação

http://centaur.reading.ac.uk/27343/

creatorInternal Rattray, Marcus

10.1186/1471-2202-13-38

Tipo

Article

PeerReviewed