Integrated functional genomics for improved manufacture of recombinant enveloped virus

Dissertation presented to obtain the Ph.D degree in Engineering and Technology Sciences-Biotechnology

Biopharmaceuticals derived from recombinant viruses comprise a plethora of prophylactic and therapeutic products from vaccines to gene therapy vectors. Retroviruses occupy an important segment of this market. Replication-defective vectors have extensively been used as delivery vehicles in gene therapy protocols. Retrovirus-like-particles have shown promising results in vaccinology as immunogen display platforms. Replication-competent particles have efficiently been used as oncolytic agents. (...)