Stoichiometric Molecularly Imprinted Polymers for the Recognition of Anti-Cancer Pro-drug Tegafur
Data(s) |
15/05/2016
31/12/1969
|
---|---|
Resumo |
Molecularly Imprinted Polymers (MIPs) targeting tegafur, an anti-cancer 5-fluorouracil pro-drug, have been prepared by stoichiometric imprinting using 2,6-bis(acrylamido)pyridine (BAAPy) as the functional monomer. Solution association between tegafur and BAAPy was studied by 1H NMR titration, which confirmed the formation of 1:1 complexes with an affinity constant of 574±15 M-1 ¬in CDCl3. Evaluation of the synthesised materials by HPLC and equilibrium rebinding experiments revealed high selectivity of the imprinted polymer for the pro-drug versus 5-fluorouracil and other competing analytes, with maximum imprinting factors of 25.3 and a binding capacity of 45.1 μmol g-1. The synthesised imprinted polymer was employed in solid-phase extraction of the pro-drug using an optimised protocol that included a simple wash with the porogen used in the preparation of the material. Tegafur recoveries of up to 96% were achieved from aqueous samples and 92% from urine samples spiked with the template and three competing analytes. The results demonstrate the potential of the prepared polymers in the pre-concentration of tegafur from biological samples, which could be an invaluable tool in the monitoring of patient compliance and drug uptake and excretion. |
Identificador | |
Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/embargoedAccess |
Fonte |
Mattos dos Santos , P , Hall , A J & Manesiotis , P 2016 , ' Stoichiometric Molecularly Imprinted Polymers for the Recognition of Anti-Cancer Pro-drug Tegafur ' Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , vol 1021 , pp. 197-203 . DOI: 10.1016/j.jchromb.2015.12.015 |
Palavras-Chave | #tegafur #5-fluorouracil #bioanalysis #molecularly imprinted polymers #solid-phase extraction #/dk/atira/pure/subjectarea/asjc/1600/1602 #Analytical Chemistry #/dk/atira/pure/subjectarea/asjc/2500/2505 #Materials Chemistry |
Tipo |
article |