Norspermidine Is Not a Self-Produced Trigger for Biofilm Disassembly


Autoria(s): Hobley, Laura; Kim, Sok Ho; Maezato, Yukari; Wyllie, Susan; Fairlamb, Alan H.; Stanley-Wall, Nicola R.; Michael, Anthony J.
Data(s)

13/02/2014

Resumo

<p>Formation of Bacillus subtilis biofilms, consisting of cells encapsulated within an extracellular matrix of exopolysaccharide and protein, requires the polyamine spermidine. A recent study reported that (1) related polyamine norspermidine is synthesized by B. subtilis using the equivalent of the Vibrio cholerae biosynthetic pathway, (2) exogenous norspermidine at 25 μM prevents B. subtilis biofilm formation, (3) endogenous norspermidine is present in biofilms at 50-80 μM, and (4) norspermidine prevents biofilm formation by condensing biofilm exopolysaccharide. In contrast, we find that, at concentrations up to 200 μM, exogenous norspermidine promotes biofilm formation. We find that norspermidine is absent in wild-type B. subtilis biofilms at all stages, and higher concentrations of exogenous norspermidine eventually inhibit planktonic growth and biofilm formation in an exopolysaccharide-independent manner. Moreover, orthologs of the V. cholerae norspermidine biosynthetic pathway are absent from B. subtilis, confirming that norspermidine is not physiologically relevant to biofilm function in this species.</p>

Formato

application/pdf

Identificador

http://pure.qub.ac.uk/portal/en/publications/norspermidine-is-not-a-selfproduced-trigger-for-biofilm-disassembly(ed73fc3b-8539-4487-9902-48190c4f2d68).html

http://dx.doi.org/10.1016/j.cell.2014.01.012

http://pure.qub.ac.uk/ws/files/15727782/Nors.pdf

Idioma(s)

eng

Direitos

info:eu-repo/semantics/openAccess

Fonte

Hobley , L , Kim , S H , Maezato , Y , Wyllie , S , Fairlamb , A H , Stanley-Wall , N R & Michael , A J 2014 , ' Norspermidine Is Not a Self-Produced Trigger for Biofilm Disassembly ' Cell , vol 156 , no. 4 , pp. 844-54 . DOI: 10.1016/j.cell.2014.01.012

Palavras-Chave #Amino Acid Sequence #Bacillus subtilis #Biofilms #Molecular Sequence Data #Plankton #Sequence Alignment #Spermidine #Vibrio cholerae #gamma-Aminobutyric Acid
Tipo

article