Preparation of planar retinal specimens:verification by histology, mRNA profiling, and proteome analysis
| Data(s) |
29/03/2004
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| Resumo |
Elucidation of the transcriptome and proteome of the normal retina will be difficult since it is comprised of at least 55 different cell types. However the characteristic layered cellular anatomy of the retina makes it amenable to planar sectioning, enabling the generation of enriched retinal cell populations. The aim of this study was to validate a reproducible method for preparing enriched retinal layers from porcine retina. |
| Identificador | |
| Idioma(s) |
eng |
| Direitos |
info:eu-repo/semantics/restrictedAccess |
| Fonte |
McKay , G J , Campbell , L , Oliver , M , Brockbank , S , Simpson , D A C & Curry , W J 2004 , ' Preparation of planar retinal specimens : verification by histology, mRNA profiling, and proteome analysis ' Molecular Vision , vol 10 , pp. 240-7 . |
| Palavras-Chave | #Anatomy, Cross-Sectional #Animals #Chromatography, Liquid #Cryoultramicrotomy #Eye Proteins #Gene Expression #Gene Expression Profiling #Mass Spectrometry #Oligonucleotide Array Sequence Analysis #Peptide Fragments #Proteome #RNA, Messenger #Reproducibility of Results #Retina #Reverse Transcriptase Polymerase Chain Reaction #Specimen Handling #Swine |
| Tipo |
article |
