Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA
Data(s) |
2012
|
---|---|
Resumo |
SU-8 epoxy-based negative photoresist has been extensively employed as a structural material for fabrication of numerous biological microelectro-mechanical systems (Bio-MEMS) or lab-on-a-chip (LOC) devices. However, SU-8 has a high autofluorescence level that limits sensitivity of microdevices that use fluorescence as the predominant detection workhorse. Here, we show that deposition of a thin gold nanoparticles layer onto the SU-8 surface significantly reduces the autofluorescence of the coated SU-8 surface by as much as 81% compared to bare SU-8. Furthermore, DNA probes can easily be immobilized on the Au surface with high thermal stability. These improvements enabled sensitive DNA detection by simple DNA hybridization down to 1 nM (a two orders of magnitude improvement) or by solid-phase PCR with sub-picomolar sensitivity. The approach is simple and easy to perform, making it suitable for various Bio-MEMs and LOC devices that use SU-8 as a structural material. |
Identificador | |
Idioma(s) |
eng |
Direitos |
info:eu-repo/semantics/restrictedAccess |
Fonte |
Cao , C , Birtwell , S W , Høgberg , J , Morgan , H , Wolff , A & Bang , D D 2012 , ' Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA ' Diagnostics , vol 2 , no. 4 , pp. 72-82 . |
Tipo |
article |