Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay


Autoria(s): Kavanagh, Owen; Estes, Mary K; Reeck, Amanda; Raju, Ravikiran M; Opekun, Antone R; Gilger, Mark A; Graham, David Y; Atmar, Robert L
Data(s)

01/07/2011

Resumo

A quantitative duplex time-resolved fluorescence assay, dissociation-enhanced lanthanide fluorescent immunoassay (DELFIA), was developed to measure Norwalk virus (NV)-specific IgA and IgG antibodies simultaneously. The duplex assay showed superior performance by detecting seroconversion following experimental NV infection at an earlier time point than a reference total immunoglobulin enzyme-linked immunosorbent assay (ELISA).

Identificador

http://pure.qub.ac.uk/portal/en/publications/serological-responses-to-experimental-norwalk-virus-infection-measured-using-a-quantitative-duplex-timeresolved-fluorescence-immunoassay(bcc4d7f7-6079-4286-9a65-186b10dd2ce1).html

http://dx.doi.org/10.1128/CVI.00039-11

Idioma(s)

eng

Direitos

info:eu-repo/semantics/restrictedAccess

Fonte

Kavanagh , O , Estes , M K , Reeck , A , Raju , R M , Opekun , A R , Gilger , M A , Graham , D Y & Atmar , R L 2011 , ' Serological responses to experimental Norwalk virus infection measured using a quantitative duplex time-resolved fluorescence immunoassay ' Clinical and Vaccine Immunology , vol 18 , no. 7 , pp. 1187-90 . DOI: 10.1128/CVI.00039-11

Palavras-Chave #Immunoglobulin A #Antibodies, Viral #Caliciviridae Infections #Serologic Tests #Norwalk virus #Humans #Immunoglobulin G #Enzyme-Linked Immunosorbent Assay #Fluoroimmunoassay #Time Factors
Tipo

article